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plvx puro plasmids  (TaKaRa)


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    Structured Review

    TaKaRa plvx puro plasmids
    Plvx Puro Plasmids, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 417 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plvx+puro+plasmids/Lenti-X+Expression+System/us12595302-545-35-37
    Average 96 stars, based on 417 article reviews
    plvx puro plasmids - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Over Expression:

    Article Title: LINC02086 promotes cell viability and inhibits cell apoptosis in breast cancer by sponging miR-6757-5p and up-regulating EPHA2.
    Article Snippet: .. For LINC02086 overexpression, the coding sequence was cloned into pLVX-Puro plasmids (Clontech, USA). .. The miRNAs used in the study (Genepharm Technologies, Shanghai, China) were listed as follows: miR-6757-5p mimic (5′-AGU AGG ACC GGA GGG UAG GGAU-3′), miR-6757-5p inhibitor (5′-UCA UCC UGG CCU CCC AUC CCUA-3′), and negative control (5′-UGAGC AAG GGC GAG GAG CUG UUC -3′).Transfection was performed using Lipofectamine 2000 reagent.

    Article Title: CLDN18.2 binding molecule
    Article Snippet: .. Construction and Identification of Overexpression Cell Lines The nucleic acid sequences of full-length human CLDN18.1 (SEQ ID NO: 16), mouse CLDN18.2 (SEQ ID NO: 17), and mouse CLDN18.1 (SEQ ID NO: 18) were constructed into pLVX-puro plasmids (Clontech, Cat #632164). .. Then the resulting plasmids were transformed into HEK293 cells (ATCC® CRL-1573TM) via electrotransformation.

    Article Title: LINC02086 promotes cell viability and inhibits cell apoptosis in breast cancer by sponging miR-6757-5p and up-regulating EPHA2
    Article Snippet: .. For LINC02086 overexpression, the coding sequence was cloned into pLVX-Puro plasmids (Clontech, USA). .. The miRNAs used in the study (Genepharm Technologies, Shanghai, China) were listed as follows: miR-6757-5p mimic (5′-AGUAGGACCGGAGGGUAGGGAU-3′), miR-6757-5p inhibitor (5′-UCAUCCUGGCCUCCCAUCCCUA-3′), and negative control (5′-UGAGC AAGGGCGAGGAGCUGUUC-3′).Transfection was performed using Lipofectamine 2000 reagent.

    Sequencing:

    Article Title: LINC02086 promotes cell viability and inhibits cell apoptosis in breast cancer by sponging miR-6757-5p and up-regulating EPHA2.
    Article Snippet: .. For LINC02086 overexpression, the coding sequence was cloned into pLVX-Puro plasmids (Clontech, USA). .. The miRNAs used in the study (Genepharm Technologies, Shanghai, China) were listed as follows: miR-6757-5p mimic (5′-AGU AGG ACC GGA GGG UAG GGAU-3′), miR-6757-5p inhibitor (5′-UCA UCC UGG CCU CCC AUC CCUA-3′), and negative control (5′-UGAGC AAG GGC GAG GAG CUG UUC -3′).Transfection was performed using Lipofectamine 2000 reagent.

    Article Title: HDAC/H3K27ac-mediated transcription of NDUFA3 exerts protective effects on high glucose-treated human nucleus pulposus cells through improving mitochondrial function.
    Article Snippet: .. Production of lentiviral vectors and cell transduction To overexpress NDUFA3, the specific coding sequence was synthesized and cloned into pLVX-Puro plasmids (Clontech Laboratories). .. To knock down NDUFA3, the specific shRNA sequences targeting NDUFA3 (shNDUFA3-1 5′-CAA GAA TGC CTG GGA CAA GGA-3′; shNDUFA3-2, 5′-GCT GGT CGT GTC CTT CGT CGT-3′; and shNDUFA3-3, 5′-CTA CTT CAA GTA CTC CGT CAT-3′) or scramble shRNA (5′-TCG AGG CTG GTG CAG CAT CAT-3′) were synthesized by Sangon Biotech (Shanghai, China) and cloned into linearized pLKO.1 plasmids (Addgene).

    Article Title: ST3GAL4 promotes tumorigenesis in breast cancer by enhancing aerobic glycolysis.
    Article Snippet: Sialyltransferases are enzymes that play a crucial role in regulating cancer progression by modifying glycoproteins through sialylation.. In particular, the ST3 beta-galactoside alpha-2,3-sialyltransferase 4 (ST3GAL4) enzyme is known to be upregulated in breast cancer, but its specific biological functions have not been fully understood.. This study aimed to investigate the impact and mechanisms of ST3GAL4 on aerobic glycolysis in breast cancer.

    Article Title: LINC02086 promotes cell viability and inhibits cell apoptosis in breast cancer by sponging miR-6757-5p and up-regulating EPHA2
    Article Snippet: .. For LINC02086 overexpression, the coding sequence was cloned into pLVX-Puro plasmids (Clontech, USA). .. The miRNAs used in the study (Genepharm Technologies, Shanghai, China) were listed as follows: miR-6757-5p mimic (5′-AGUAGGACCGGAGGGUAGGGAU-3′), miR-6757-5p inhibitor (5′-UCAUCCUGGCCUCCCAUCCCUA-3′), and negative control (5′-UGAGC AAGGGCGAGGAGCUGUUC-3′).Transfection was performed using Lipofectamine 2000 reagent.

    Clone Assay:

    Article Title: LINC02086 promotes cell viability and inhibits cell apoptosis in breast cancer by sponging miR-6757-5p and up-regulating EPHA2.
    Article Snippet: .. For LINC02086 overexpression, the coding sequence was cloned into pLVX-Puro plasmids (Clontech, USA). .. The miRNAs used in the study (Genepharm Technologies, Shanghai, China) were listed as follows: miR-6757-5p mimic (5′-AGU AGG ACC GGA GGG UAG GGAU-3′), miR-6757-5p inhibitor (5′-UCA UCC UGG CCU CCC AUC CCUA-3′), and negative control (5′-UGAGC AAG GGC GAG GAG CUG UUC -3′).Transfection was performed using Lipofectamine 2000 reagent.

    Article Title: HDAC/H3K27ac-mediated transcription of NDUFA3 exerts protective effects on high glucose-treated human nucleus pulposus cells through improving mitochondrial function.
    Article Snippet: .. Production of lentiviral vectors and cell transduction To overexpress NDUFA3, the specific coding sequence was synthesized and cloned into pLVX-Puro plasmids (Clontech Laboratories). .. To knock down NDUFA3, the specific shRNA sequences targeting NDUFA3 (shNDUFA3-1 5′-CAA GAA TGC CTG GGA CAA GGA-3′; shNDUFA3-2, 5′-GCT GGT CGT GTC CTT CGT CGT-3′; and shNDUFA3-3, 5′-CTA CTT CAA GTA CTC CGT CAT-3′) or scramble shRNA (5′-TCG AGG CTG GTG CAG CAT CAT-3′) were synthesized by Sangon Biotech (Shanghai, China) and cloned into linearized pLKO.1 plasmids (Addgene).

    Article Title: ST3GAL4 promotes tumorigenesis in breast cancer by enhancing aerobic glycolysis.
    Article Snippet: Sialyltransferases are enzymes that play a crucial role in regulating cancer progression by modifying glycoproteins through sialylation.. In particular, the ST3 beta-galactoside alpha-2,3-sialyltransferase 4 (ST3GAL4) enzyme is known to be upregulated in breast cancer, but its specific biological functions have not been fully understood.. This study aimed to investigate the impact and mechanisms of ST3GAL4 on aerobic glycolysis in breast cancer.

    Article Title: Sacituzumab tirumotecan in advanced non-small-cell lung cancer with or without EGFR mutations: phase 1/2 and phase 2 trials.
    Article Snippet: Trophoblast cell-surface antigen 2 (TROP2)-directed antibody–drug conjugate (ADC) is a promising anticancer agent that has shown remarkable efficacy in several malignancies.. However, in lung cancer, two phase 3 trials on TROP2-ADCs in unselected patients with advanced non-small-cell lung cancer (NSCLC) have both failed.. Sacituzumab tirumotecan (sac-TMT) is a novel TROP2-directed ADC.

    Article Title: LINC02086 promotes cell viability and inhibits cell apoptosis in breast cancer by sponging miR-6757-5p and up-regulating EPHA2
    Article Snippet: .. For LINC02086 overexpression, the coding sequence was cloned into pLVX-Puro plasmids (Clontech, USA). .. The miRNAs used in the study (Genepharm Technologies, Shanghai, China) were listed as follows: miR-6757-5p mimic (5′-AGUAGGACCGGAGGGUAGGGAU-3′), miR-6757-5p inhibitor (5′-UCAUCCUGGCCUCCCAUCCCUA-3′), and negative control (5′-UGAGC AAGGGCGAGGAGCUGUUC-3′).Transfection was performed using Lipofectamine 2000 reagent.

    Transduction:

    Article Title: HDAC/H3K27ac-mediated transcription of NDUFA3 exerts protective effects on high glucose-treated human nucleus pulposus cells through improving mitochondrial function.
    Article Snippet: .. Production of lentiviral vectors and cell transduction To overexpress NDUFA3, the specific coding sequence was synthesized and cloned into pLVX-Puro plasmids (Clontech Laboratories). .. To knock down NDUFA3, the specific shRNA sequences targeting NDUFA3 (shNDUFA3-1 5′-CAA GAA TGC CTG GGA CAA GGA-3′; shNDUFA3-2, 5′-GCT GGT CGT GTC CTT CGT CGT-3′; and shNDUFA3-3, 5′-CTA CTT CAA GTA CTC CGT CAT-3′) or scramble shRNA (5′-TCG AGG CTG GTG CAG CAT CAT-3′) were synthesized by Sangon Biotech (Shanghai, China) and cloned into linearized pLKO.1 plasmids (Addgene).

    Synthesized:

    Article Title: HDAC/H3K27ac-mediated transcription of NDUFA3 exerts protective effects on high glucose-treated human nucleus pulposus cells through improving mitochondrial function.
    Article Snippet: .. Production of lentiviral vectors and cell transduction To overexpress NDUFA3, the specific coding sequence was synthesized and cloned into pLVX-Puro plasmids (Clontech Laboratories). .. To knock down NDUFA3, the specific shRNA sequences targeting NDUFA3 (shNDUFA3-1 5′-CAA GAA TGC CTG GGA CAA GGA-3′; shNDUFA3-2, 5′-GCT GGT CGT GTC CTT CGT CGT-3′; and shNDUFA3-3, 5′-CTA CTT CAA GTA CTC CGT CAT-3′) or scramble shRNA (5′-TCG AGG CTG GTG CAG CAT CAT-3′) were synthesized by Sangon Biotech (Shanghai, China) and cloned into linearized pLKO.1 plasmids (Addgene).

    Article Title: ST3GAL4 promotes tumorigenesis in breast cancer by enhancing aerobic glycolysis.
    Article Snippet: Sialyltransferases are enzymes that play a crucial role in regulating cancer progression by modifying glycoproteins through sialylation.. In particular, the ST3 beta-galactoside alpha-2,3-sialyltransferase 4 (ST3GAL4) enzyme is known to be upregulated in breast cancer, but its specific biological functions have not been fully understood.. This study aimed to investigate the impact and mechanisms of ST3GAL4 on aerobic glycolysis in breast cancer.

    Construct:

    Article Title: CLDN18.2 binding molecule
    Article Snippet: .. Construction and Identification of Overexpression Cell Lines The nucleic acid sequences of full-length human CLDN18.1 (SEQ ID NO: 16), mouse CLDN18.2 (SEQ ID NO: 17), and mouse CLDN18.1 (SEQ ID NO: 18) were constructed into pLVX-puro plasmids (Clontech, Cat #632164). .. Then the resulting plasmids were transformed into HEK293 cells (ATCC® CRL-1573TM) via electrotransformation.

    Article Title: Loss of BACH1 improves osteogenic differentiation in glucocorticoid-induced hBMSCs through restoring autophagy
    Article Snippet: The short hairpin RNAs (shRNAs) against for BACH1 (shBACH1) and ATG7 (shATG7), as well as their negative control (shNC) were all customized ordered from Genscript (Nanjing, China). .. Then, the above shRNAs were inserted into pLVX-Puro plasmids (Takara, Tokyo, Japan) to construct recombinant plasmids. ..

    Article Title: Loss of BACH1 improves osteogenic differentiation in glucocorticoid-induced hBMSCs through restoring autophagy.
    Article Snippet: After washing by PBS, cells were incubated with secondary goat Anti-Rabbit IgG H&L, Alexa Fluor® 488) (Abcam, Cat#ab150077) for 1 h. Fluorescence images from 5 fields/well were scanned using an automated microscope IN Cell Analyzer 6000 (GE Healthcare, Bucks, UK). shRNA for gene knockdown The short hairpin RNAs (shRNAs) against for BACH1 (shBACH1) and ATG7 (shATG7), as well as their negative control (shNC) were all customized ordered from Genscript (Nanjing, China). .. Then, the above shRNAs were inserted into pLVX-Puro plasmids (Takara, Tokyo, Japan) to construct recombinant plasmids. ..

    Recombinant:

    Article Title: Loss of BACH1 improves osteogenic differentiation in glucocorticoid-induced hBMSCs through restoring autophagy
    Article Snippet: The short hairpin RNAs (shRNAs) against for BACH1 (shBACH1) and ATG7 (shATG7), as well as their negative control (shNC) were all customized ordered from Genscript (Nanjing, China). .. Then, the above shRNAs were inserted into pLVX-Puro plasmids (Takara, Tokyo, Japan) to construct recombinant plasmids. ..

    Article Title: Loss of BACH1 improves osteogenic differentiation in glucocorticoid-induced hBMSCs through restoring autophagy.
    Article Snippet: After washing by PBS, cells were incubated with secondary goat Anti-Rabbit IgG H&L, Alexa Fluor® 488) (Abcam, Cat#ab150077) for 1 h. Fluorescence images from 5 fields/well were scanned using an automated microscope IN Cell Analyzer 6000 (GE Healthcare, Bucks, UK). shRNA for gene knockdown The short hairpin RNAs (shRNAs) against for BACH1 (shBACH1) and ATG7 (shATG7), as well as their negative control (shNC) were all customized ordered from Genscript (Nanjing, China). .. Then, the above shRNAs were inserted into pLVX-Puro plasmids (Takara, Tokyo, Japan) to construct recombinant plasmids. ..



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